Coal Geology & Exploration
Abstract
Background The core of coalbed methane (CBM) bioengineering is to inject bio-fracturing fluids rich in efficient methanogenic bacteria into coal reservoirs to promote the conversion of coals to methane. However, the relationships of the hydrochemical characteristics of coal seam water with the structures and metabolic functions of microbial communities remain unclear. Methods This study investigated coal seam water from 12 typical mining areas in Ningxia. Based on microbial classification and sequencing, as well as statistical analysis, this study explored the relationships of pH, anions, cations, and chemical oxygen demand (COD) of coal seam water with the structures and metabolic functions of microbial communities. Results and Conclusions The results indicate that the bacterial communities consist primarily of hydrolytic bacteria and bacteria enabling acidogenic fermentation, with dominant genera including Proteiniborus, Clostridium_sensu_stricto_1, and Thauera. The metabolism of methanogenic archaea occurs primarily through mixotrophy, with Methanosarcina identified as the dominant genus of these archaea. The coal seam water exhibits pH values ranging from 7.4 to 8.5. A decrease in pH value corresponds to increased diversity of the bacterial communities. However, the pH value exerts a small impact on the archaeal communities and an insignificant impact on the microbial metabolic functions. The diversity and abundance of the bacterial communities are positively correlated with the mass concentrations of anions Cl– and ${\mathrm{SO}}_4^{2-} $ when their mass concentrations are less than 905 mg/L and 1 974 mg/L, respectively. In contrast, the mass concentrations of cations Ca2+ and Mg2+ are significantly negatively correlated with microbial cell motility, intracellular transport, secretion, and vesicular transport, as well as the metabolism of inorganic ions, when their mass concentrations range from 5.6 mg/L to 411.0 mg/L and from 30.3 mg/L to 697.0 mg/L, respectively. Additionally, the COD in coal seam water exhibits significant positive correlations with the energy generation and transformation, along with the carbon cycle, involving microbial communities. Higher COD is associated with richer organic matter in water, thereby enhancing the involvement of microbial communities in the carbon cycle. The results of this study reveal the mechanisms behind the impacts of the hydrochemical characteristics of coal seam water on microbial communities while also laying a scientific basis for optimizing the formulation of bio-fracturing fluids in CBM bioengineering. Moreover, these results help understand the potential impacts of environmental changes on underground microbial communities during CBM production.
Keywords
coal seam water, microbial community, hydrochemical characteristics, cellular metabolism, methanogenic bacteria, coalbed methane (CBM) bioengineering
DOI
10.12363/issn.1001-1986.24.11.0691
Recommended Citation
WANG Gaohao, DUAN Shengjie, WANG Bei,
et al.
(2025)
"Impacts of the hydrochemical characteristics of coal seam water on the structures and metabolic functions of microbial communities,"
Coal Geology & Exploration: Vol. 53:
Iss.
5, Article 10.
DOI: 10.12363/issn.1001-1986.24.11.0691
Available at:
https://cge.researchcommons.org/journal/vol53/iss5/10
Reference
[1] 唐书恒,李洋,吕建伟. 原位储层生物地球化学评价及其对煤层气开采的指示意义:以沁水盆地南部柿庄南区块为例[J]. 煤炭学报,2024,49(1):555−562.
TANG Shuheng,LI Yang,LYU Jianwei. In situ reservoir biogeochemical evaluation and its indicative significance for coalbed methane extraction:Taking the Shizhuangnan Block in the southern Qinshui Basin as an example[J]. Journal of China Coal Society,2024,49(1):555−562.
[2] DONG Hailiang,HUANG Liuqin,ZHAO Linduo,et al. A critical review of mineral–microbe interaction and co–evolution:Mechanisms and applications[J]. National Science Review,2022,9(10):128.
[3] LI Saisai,XIA Daping,Chen Zhenhong,et al. Experimental study on the change of coal structure and microbial community structure during supercritical-CO2-H2O-microorganisms-coal interaction process[J]. Environmental Technology & Innovation,2023,30:103036.
[4] 聂志强,杨秀清,韩作颖. 不同煤阶生物成因煤层气微生物群落的功能及多样性研究进展[J]. 微生物学通报,2019,46(5):1127−1135.
NIE Zhiqiang,YANG Xiuqing,HAN Zuoying. Function and diversity of microbial community in biogenic coal–bed methane with different coal ranks:A review[J]. Microbiology China,2019,46(5):1127−1135.
[5] 董海良,曾强,刘邓,等. 黏土矿物–微生物相互作用机理以及在环境领域中的应用[J]. 地学前缘,2024,31(1):467−485.
DONG Hailiang,ZENG Qiang,LIU Deng,et al. Interactions between clay minerals and microbes:Mechanisms and applications in environmental remediation[J]. Earth Science Frontiers,2024,31(1):467−485.
[6] SHIMIZU S,AKIYAMA M,NAGANUMA T,et al. Molecular characterization of microbial communities in deep coal seam groundwater of northern Japan[J]. Geobiology,2007,5(4):423−433.
[7] 苏现波,夏大平,赵伟仲,等. 煤层气生物工程研究进展[J]. 煤炭科学技术,2020,48(6):1−30.
SU Xianbo,XIA Daping,ZHAO Weizhong,et al. Research advances of coalbed gas bioengineering[J]. Coal Science and Technology,2020,48(6):1−30.
[8] 杨秀清,吴瑞薇,韩作颖,等. 基于mcrA基因的沁水盆地煤层气田产甲烷菌群与途径分析[J]. 微生物学通报,2017,44(4):795−806.
YANG Xiuqing,WU Ruiwei,HAN Zuoying,et al. Analysis of methanogenic community and pathway of coalbed methane fields in the Qinshui Basin based on mcrA gene[J]. Microbiology China,2017,44(4):795−806.
[9] 刘亚飞,王波波,张洪勋,等. 芦岭煤田微生物群落结构和生物成因气的产甲烷类型研究[J]. 微生物学报,2019,59(6):1174−1187.
LIU Yafei,WANG Bobo,ZHANG Hongxun,et al. Microbial community and the type of methanogenesis associated with biogenic gas in Luling Coalfield,China[J]. Acta Microbiologica Sinica,2019,59(6):1174−1187.
[10] 张博雅,余珂. 微生物基因数据库在氮循环功能基因注释中的应用[J]. 微生物学通报,2020,47(9):3021−3038.
ZHANG Boya,YU Ke. Application of microbial gene databases in the annotation of nitrogen cycle functional genes[J]. Microbiology China,2020,47(9):3021−3038.
[11] 张宏,李颖杰,王文颖,等. 微生物硫循环网络的研究进展[J]. 微生物学报,2021,61(6):1567−1581.
ZHANG Hong,LI Yingjie,WANG Wenying,et al. Research progress of the microbial sulfur–cycling network[J]. Acta Microbiologica Sinica,2021,61(6):1567−1581.
[12] PYTLAK A,JAROMIN–GLEŃ K,SUJAK A,et al. Interactions between surface properties of pristine coals and the intrinsic microbial communities involved in methane formation[J]. International Journal of Coal Geology,2024,282:104422.
[13] 易悦,周卓,黄艳,等. 我国产甲烷古菌研究进展与展望[J]. 微生物学报,2023,63(5):1796−1814.
YI Yue,ZHOU Zhuo,HUANG Yan,et al. Methanogen research in China:Current status and prospective[J]. Acta Microbiologica Sinica,2023,63(5):1796−1814.
[14] 承磊,郑珍珍,王聪,等. 产甲烷古菌研究进展[J]. 微生物学通报,2016,43(5):1143−1164.
CHENG Lei,ZHENG Zhenzhen,WANG Cong,et al. Recent advances in methanogens[J]. Microbiology China,2016,43(5):1143−1164.
[15] CAI Mingwei,YIN Xiuran,TANG Xiaoyu,et al. Metatranscriptomics reveals different features of methanogenic archaea among global vegetated coastal ecosystems[J]. Science of the Total Environment,2022,802:149848.
[16] 任师杰,孔令豆,刘骏,等. 产甲烷古菌的分类及代谢途径研究进展[J]. 中国生物工程杂志,2024,44(9):100−112.
REN Shijie,KONG Lingdou,LIU Jun,et al. Advances in classification and metabolic pathways of methanogenic archaea[J]. China Biotechnology,2024,44(9):100−112.
[17] JETTEN M S M,STAMS A J M,ZEHNDER A J B. Methanogenesis from acetate:A comparison of the acetate metabolism in Methanothrix soehngenii and Methanosarcina spp.[J]. FEMS Microbiology Letters,1992,88(3/4):181−197.
[18] ROTARU A E,SHRESTHA P M,LIU Fanghua,et al. Direct interspecies electron transfer between Geobacter metallireducens and Methanosarcina barkeri[J]. Applied and Environmental Microbiology,2014,80(15):4599−4605.
[19] GARETH J,JOHNSON. Encyclopedia of microbiology[J]. Reference Reviews,1997.
[20] 张娜,尹雪峰,王子琛,等. 微生物增产煤层气作用机理及影响因素研究进展[J]. 过程工程学报,2024,24(6):636−646.
ZHANG Na,YIN Xuefeng,WANG Zichen,et al. Research progress on the mechanism and influencing factors of microorganisms to increase coalbed methane production[J]. The Chinese Journal of Process Engineering,2024,24(6):636−646.
[21] 左照江,张汝民,高岩. 盐胁迫下植物细胞离子流变化的研究进展[J]. 浙江农林大学学报,2014,31(5):805−811.
ZUO Zhaojiang,ZHANG Rumin,GAO Yan. Advances in plant cell ion flux with salt stress:A review[J]. Journal of Zhejiang A & F University,2014,31(5):805−811.
[22] 庞安冉,张晓丹,刘淼,等. 不同pH值条件下硫酸盐还原菌组成及硫酸盐还原机制分析[J]. 微生物学报,2024,64(4):1081−1094.
PANG Anran,ZHANG Xiaodan,LIU Miao,et al. Dominant sulfate–reducing bacteria at different pH and mechanism of sulfate reduction[J]. Acta Microbiologica Sinica,2024,64(4):1081−1094.
[23] ZHANG Zhao,ZHANG Chunhui,YANG Yang,et al. A review of sulfate–reducing bacteria:Metabolism,influencing factors and application in wastewater treatment[J]. Journal of Cleaner Production,2022,376:134109.
[24] QIU Shuang,ZHANG Xingchen,XIA Wenhao,et al. Effect of extreme pH conditions on methanogenesis:Methanogen metabolism and community structure[J]. Science of the Total Environment,2023,877:162702.
[25] 李子祥,奕栋,姜水琴,等. 氧化还原电位在微生物发酵中的应用[J]. 中国酿造,2024,43(5):25−31.
LI Zixiang,YI Dong,JIANG Shuiqin,et al. Application of oxidation–reduction potential in microbial fermentation[J]. China Brewing,2024,43(5):25−31.
[26] 汤伟,张军,李广善,等. 深海极端微生物菌群及代谢产物多样性的研究进展[J]. 微生物学报,2019,59(7):1241−1252.
TANG Wei,ZHANG Jun,LI Guangshan,et al. Diversity of extremophiles and metabolites in the deep–sea[J]. Acta Microbiologica Sinica,2019,59(7):1241−1252.
[27] 刘海昌,兰贵红,刘全全,等. 高温油藏采出液中嗜热产甲烷古菌的分离鉴定[J]. 生物工程学报,2010,26(7):1009−1013.
LIU Haichang,LAN Guihong,LIU Quanquan,et al. Isolation and identification of a methanogen from the high temperature oil reservoir water[J]. Chinese Journal of Biotechnology,2010,26(7):1009−1013.
[28] 张莉,徐智敏,孙亚军,等. 鄂尔多斯典型煤矿不同功能区水化学与微生物群落特征及环境响应[J]. 煤炭科学技术,2023,51(12):180−196.
ZHANG Li,XU Zhimin,SUN Yajun,et al. Hydrochemistry and microbial community characteristics and environmental response in different functional zones of a typical coal mine in Ordos[J]. Coal Science and Technology,2023,51(12):180−196.
[29] 范立民,李涛,高颖,等. 生态脆弱煤矿区水体中微生物群落特征及矿井充水指示[J]. 煤炭科学技术,2024,52(1):255−266.
FAN Limin,LI Tao,GAO Ying,et al. Characteristics of microbial communities in water bodies of ecologically fragile coal mining areas and indications for mine water filling[J]. Coal Science and Technology,2024,52(1):255−266.
[30] LI Xiaoli,SONG Lai,WANG Guoliang,et al. Complete genome sequence of a deeply branched marine Bacteroidia bacterium Draconibacterium orientale type strain FH5T[J]. Marine Genomics,2016,26:13−16.
[31] MAKI J J,NIELSEN D W,LOOFT T. Complete genome sequence and annotation for Romboutsia sp. Strain CE17[J]. Microbiology Resource Announcements,2020,9(23):e0038220.
[32] PESANTE G,TESORIERO C,CADORIA E,et al. Valorisation of agricultural residues into Thauera sp. Sel9 microbial proteins for aquaculture[J]. Environmental Technology & Innovation,2024,36:103772.
[33] HÖRDT A,LÓPEZ M G,MEIER–KOLTHOFF J P,et al. Analysis of 1,000+ type–strain genomes substantially improves taxonomic classification of Alphaproteobacteria[J]. Frontiers in Microbiology,2020,11:468.
[34] FAN Zijian,KE Xiaoli,JIANG Lijin,et al. Genomic and biochemical analysis reveals fermented product of a putative novel Romboutsia species involves the glycometabolism of tilapia[J]. Aquaculture,2024,581:740483.
[35] CAO Hongrui,SUN Jin,WANG Keqiang,et al. Performance of bioelectrode based on different carbon materials in bioelectrochemical anaerobic digestion for methanation of maize straw[J]. Science of the Total Environment,2022,832:154997.
[36] 张莉,徐智敏,孙亚军,等. 煤矿矿井水水质形成及演化的水化学–微生物场作用及数学模型构建[J]. 中国矿业大学学报,2024,53(5):943−959.
ZHANG Li,XU Zhimin,SUN Yajun,et al. Hydrochemical–microbial field interaction and mathematical model construction of the water quality formation and evolution in coal mine[J]. Journal of China University of Mining & Technology,2024,53(5):943−959.
[37] QIAN Youfen,XU Meiying,DENG Tongchu,et al. Synergistic interactions of Desulfovibrio and Petrimonas for sulfate–reduction coupling polycyclic aromatic hydrocarbon degradation[J]. Journal of Hazardous Materials,2021,407:124385.
[38] SÁNCHEZ–SÁNCHEZ C,ARANDA–MEDINA M,RODRÍGUEZ A,et al. Development of real–time PCR methods for the quantification of Methanoculleus,Methanosarcina and Methanobacterium in anaerobic digestion[J]. Journal of Microbiological Methods,2022,199:106529.
[39] CHEN Dan,PEI Haoyi,ZHOU Ningli,et al. CO2 reduction to CH4 by Methanosarcina barkeri and a mixed methanogenic culture using humin as sole electron donor[J]. Energy,2024,294:130841.
[40] MA Xiaobiao,JI Jing,SONG Peizhi,et al. Treatment of nanofiltration membrane concentrates integrated magnetic biochar pretreatment with anaerobic digestion[J]. Environmental Research,2023,221:115245.
Included in
Earth Sciences Commons, Mining Engineering Commons, Oil, Gas, and Energy Commons, Sustainability Commons