Coal Geology & Exploration


In order to resolve the issue of soil degradation in the dumping site of open-pit coal mine in arid area, the effects of actinomycetes on the root growth and rhizosphere soil fertility of onobrychis viciaefolia in the dumping site of Xinjiang Heishan Open-pit Coal Mine were investigated by highly efficient actinomycete inoculation and in-situ pot experiment. The results show that: (1) Under the drought conditions, actinomycetes inoculation could significantly promote the development of root morphology and the growth of onobrychis viciaefolia. Compared with the control without actinomycetes inoculation, the total root length, average root diameter, root surface area and total root volume of onobrychis viciaefolia were significantly increased (P<0.05), and the fresh weight of root and stem leaf and the total fresh weight were increased by 77.24%, 130% and 103.49% respectively. (2) Actinomycetes inoculation significantly enhanced the drought resistance of onobrychis viciaefolia. Meanwhile, the root glutathione, proline content and root activity were increased by 35.36%, 229.23% and 363.75% respectively, while malondialdehyde content and the permeability of the root cytoplasmic membrane were reduced. (3) Actinomycetes inoculation significantly improved the rhizosphere soil fertility of onobrychis viciaefolia. Specifically, the colonization amount of actinomycetes for test in rhizosphere soil of onobrychis viciaefolia is 2.5 × 105 cfu/g. The total number of soil bacteria and actinomycetes in the rhizosphere were increased by 113.7% and 563.64% respectively. Besides, the activities of soil polyphenol oxidase, urease and alkaline phosphatase were significantly increased (P<0.05) by comparison with the control. Thus, the contents of total nitrogen, available phosphorus and available potassium in rhizosphere soil were significantly increased by actinomycetes, of which the total nitrogen reached the largest increase rate. (4) Correlation analysis shows that root biomass of onobrychis viciaefolia was significantly and positively correlated with the quantity of soil bacteria and actinobacteria, the activity of urease and alkaline phosphatase, and the total nitrogen (P<0.01). These results also verify that actinomycetes inoculation in soil could enhance the drought resistance of plant, increase the number of microorganisms, enzyme activity and fertility in rhizosphere soil, and significantly promote the development and growth of plant root, thus providing scientific basis for vegetation restoration and degraded soil improvement in arid coal mining areas.


arid area,open-pit coal mine,actinomycetes,onobrychis viciaefolia,root growth characteristic,soil fertility,mine ecological restoration




[1] 申艳军,杨博涵,王双明,等. 黄河几字弯区煤炭基地地质灾害与生态环境典型特征[J]. 煤田地质与勘探,2022,50(6):104−117.

SHEN Yanjun,YANG Bohan,WANG Shuangming,et al. Typical characteristics of geological hazards and ecological environment of coal base in the bends area of the Yellow River[J]. Coal Geology & Exploration,2022,50(6):104−117.

[2] 毕银丽,彭苏萍,杜善周. 西部干旱半干旱露天煤矿生态重构技术难点及发展方向[J]. 煤炭学报,2021,46(5):1355−1364.

BI Yinli,PENG Suping,DU Shanzhou. Technological difficulties and future directions of ecological reconstruction in open pit coal mine of the arid and semi−arid areas of western China[J]. Journal of China Coal Society,2021,46(5):1355−1364.

[3] 卞正富,于昊辰,侯竟,等. 西部重点煤矿区土地退化的影响因素及其评估[J]. 煤炭学报,2020,45(1):338−350.

BIAN Zhengfu,YU Haochen,HOU Jing,et al. Influencing factors and evaluation of land degradation of 12 coal mine areas in western China[J]. Journal of China Coal Society,2020,45(1):338−350.

[4] BI Yinli,ZHANG Yanxu,ZOU Hui. Plant growth and their root development after inoculation of arbuscular mycorrhizal fungi in coal mine subsided areas[J]. International Journal of Coal Science and Technology,2018,5:47−53.

[5] LI Junqin,MENG Bo,CHAI Hua,et al. Arbuscular mycorrhizal fungi alleviate drought stress in C3 (Leymus chinensis) and C4 (Hemarthria altissima) grasses via altering antioxidant enzyme activities and photosynthesis[J]. Frontiers in Plant Science,2019,10:499.

[6] SONG Ziheng,BI Yinli,ZHANG Jian,et al. Arbuscular mycorrhizal fungi promote the growth of plants in the mining associated clay[J]. Scientific Reports,2020,10:2663.

[7] 毕银丽,彭苏萍,王淑惠. 西部煤矿区深色有隔内生真菌修复机理与生态应用模式[J]. 煤炭学报,2022,47(1):460−469.

BI Yinli,PENG Suping,WANG Shuhui. Restoration mechanism and ecological application model of dark septate endophytic fungi in western mining area[J]. Journal of China Coal Society,2022,47(1):460−469.

[8] RUOTSALAINEN A L,KAUPPINEN M,WALI P R,et al. Dark septate endophytes:Mutualism from by−products?[J]. Trends in Plant Science,2021,27(3):247−254.

[9] OLANREWAJU O S,BABALOLA O O. Streptomyces:Implications and interactions in plant growth promotion[J]. Applied Microbiology and Biotechnology,2019,103(3):1179−1188.

[10] 段雪梅,赵飞扬,颜霞,等. 生防菌密旋链霉菌Act12中SPA7074缺失突变株的构建及其次级代谢产物鉴定[J]. 微生物学报,2016,56(12):1883−1891.

DUAN Xuemei,ZHAO Feiyang,YAN Xia,et al. Construction of SPA7074–deficient mutant of biocontrol strain Streptomyces pactum Act12 and characterization of its secondary metabolites[J]. Acta Microbiologica Sinica,2016,56(12):1883−1891.

[11] 马军妮,刘玉涛,李玉龙,等. 两株链霉菌对玉米的促生增产作用及机理[J]. 应用生态学报,2017,28(1):315−326.

MA Junni,LIU Yutao,LI Yulong,et al. Effects and mechanism of two Streptomyces strains on promoting plant growth and increasing grain yield of maize[J]. Chinese Journal of Applied Ecology,2017,28(1):315−326.

[12] CAO Shumiao,WANG Wenke,WANG Fei,et al. Drought–tolerant Streptomyces pactum Act12 assist phytoremediation of cadmium−contaminated soil by Amaranthus hypochondriacus:Great potential application in arid/semi−arid areas[J]. Environmental Science and Pollution Research,2016,23(15):14898−14907.

[13] LI Yulong,LI Haiyang,HAN Xue,et al. Actinobacterial biofertilizer improves the yields of different plants and alters the assembly processes of rhizosphere microbial communities[J]. Applied Soil Ecology,2022,171:104345.

[14] 李堆淑,何念武,冀玉良. 干旱胁迫下灰色链霉菌对桔梗幼苗根际土壤酶活性、养分及微生物的影响[J]. 干旱地区农业研究,2017,35(6):173−180.

LI Duishu,HE Nianwu,JI Yuliang. Effects of Streptomyces griseus on soil enzyme activity,nutrients and microorganism of the seedlings of Platycodon grandiflorum rhizosphere under drought stress[J]. Agricultural Research in the Arid Areas,2017,35(6):173−180.

[15] 何斐,张忠良,崔鸣,等. 放线菌“D74”对魔芋的防病促生作用[J]. 园艺学报,2015,42(2):367−376.

HE Fei,ZHANG Zhongliang,CUI Ming,et al. Disease prevention and growth promotion effects of actinomycete strain D74 on Amorphophallus konjac[J]. Acta Horticulturae Sinica,2015,42(2):367−376.

[16] 张志良,李小方. 植物生理学实验指导(第5版)[M]. 北京:高等教育出版社,2016.

[17] OWENS C W,BELCHER R V. A colorimetric micro–method for the determination of glutathione[J]. Biochemical Journal,1965,94(3):705−711.

[18] 关松荫. 土壤酶及其研究法[M]. 北京:农业出版社,1986.

[19] 鲍士旦,江荣风,杨超光,等. 土壤农化分析(第三版)[M]. 北京:中国农业出版社,1981.

[20] 刘建新. 不同农田土壤酶活性与土壤养分相关关系研究[J]. 土壤通报,2004,35(4):523−525.

LIU Jianxin. Correlative research on the activity of enzyme and soil nutrient in the different types of farm land[J]. Chinese Journal of Soil Science,2004,35(4):523−525.

[21] 郭小龙. 天山北坡煤矿废弃地植被恢复研究[D]. 乌鲁木齐:新疆大学,2020.

GUO Xiaolong. Study on vegetation restoration of wasteland coal mine in north slope of Tianshan Mountain[D]. Urumqi:Xinjiang University,2020.

[22] MORA–ORTIZ M,SMITH L M J. Onobrychis viciifolia:A comprehensive literature review of its history,etymology,taxonomy,genetics,agronomy and botany[J]. Plant Genetic Resources,2018,16(5):403−418.

[23] 谢开云,曹凯,万江春,等. 新疆半干旱区不同豆科/禾本科牧草混播草地生产力的变化研究[J]. 草业学报,2020,29(4):29−40.

XIE Kaiyun,CAO Kai,WAN Jiangchun,et al. Change in productivity of swards of different forage legume and grass species monocultures and combinations in the semi–arid region of Xinjiang Province[J]. Acta Prataculturae Sinica,2020,29(4):29−40.

[24] LU Bianhe,QIAN Jin,HU Jing,et al. The role of fine root morphology in nitrogen uptake by riparian plants[J]. Plant and Soil,2022,472:527−542.

[25] KONG Jing,PEI Zongping,DU Min,et al. Effects of arbuscular mycorrhizal fungi on the drought resistance of the mining area repair plant Sainfoin[J]. International Journal of Mining Science and Technology,2014,24(4):485−489.

[26] 汤东,程平,杨建军,等. 天山北坡山前植物对干旱胁迫的生理响应[J]. 干旱区研究,2021,38(6):1683−1694.

TANG Dong,CHENG Ping,YANG Jianjun,et al. Physiological responses of plants to drought stress in the northern piedmont,Tianshan Mountains[J]. Arid Zone Research,2021,38(6):1683−1694.

[27] BIDABADI S S,VANDERWEIDE J,SABBATINI P. Exogenous melatonin improves glutathione content,redox state and increases essential oil production in two Salvia species under drought stress[J]. Scientific Reports,2020,10:6883.

[28] BLUM A,EBERCON A. Genotypic responses in Sorghum to drought stress. III. free proline accumulation and drought resistance[J]. Crop Science,1976,16(3):428−431.

[29] JAMIR E,KANGABAM R D,BORAH K,et al. Role of soil microbiome and enzyme activities in plant growth nutrition and ecological restoration of soil health[M]// GONZÁLEZ-BENÍTEZ N,MOLINA M C,KINGSLEY K,et al. Microbes and Enzymes in Soil Health and Bioremediation. Singapore:Springer,2019:99–132.

[30] ZHAO Mengli,ZHAO Jun,YUAN Jun,et al. Root exudates drive soil−microbe−nutrient feedbacks in response to plant growth[J]. Plant,Cell & Environment,2021,44(2):613−628.

[31] BODNER G,MENTLER A,KEIBLINGER K M. Plant roots for sustainable soil structure management in cropping systems[M]. New York:John Wiley & Sons Inc. ,2021:45–90.

[32] FAN Lili,TARIN M W K,ZHANG Yangyang,et al. Patterns of soil microorganisms and enzymatic activities of various forest types in coastal sandy land[J]. Global Ecology and Conservation,2021,28:e01625.

[33] ISSAH G,SCHOENAU J J,LARDNER H A,et al. Nitrogen fixation and resource partitioning in alfalfa (Medicago sativa L. ),cicer milkvetch (Astragalus cicer L. ) and sainfoin (Onobrychis viciifolia Scop. ) using 15N enrichment under controlled environment conditions[J]. Agronomy,2020,10(9):1438.



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